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Denver Man
Platinum Boarder
Posts:6956
On the day I was born daddy sat down and cried

Re: Tell Me Something Good!!

#249155 1 year ago
skidoo wrote:
my b cell culture looks marvelous. flow sort for resting b cells, then plate on NIH3T3tCD40L cells (200K cells per well of a 6 well plate). split every 3rd day. culture in DMEM F12 supplemented with insulin and IL-4. you end up with activated b cells expressing CD62L, CD83, CD86. they grow like gangbusters!

now to use this as antigen-presenting cells to detect antigen-specific T cells........

and it is spring here in Seattle, beautiful night last night. had a moonlight picnic down at The Cut.


Hmmm a biotech cell culture scientist living in Seattle ... I could make a pretty good guess where you work!

PM me sometime man!
I went walkin' out last summer
Tryin' to find a breath of air
I went walkin' on the mountain
A friend had told me I'd find you there
alilfurther
Platinum Boarder
Posts:1909

Re: Tell Me Something Good!!

#249158 1 year ago
it is friggin FRIDAY!!!!! it is alllll good !!!!
Let It SHIIIINE !!!!!!!!!!
The following user(s) said Thank You: SunshineSue
skidoo
Expert Boarder
Posts:898
skidoo, skidoo.....ski-doo doo doodlie doo doo doo

Re: Tell Me Something Good!!

#249159 1 year ago
Denver Man wrote:
skidoo wrote:
my b cell culture looks marvelous. flow sort for resting b cells, then plate on NIH3T3tCD40L cells (200K cells per well of a 6 well plate). split every 3rd day. culture in DMEM F12 supplemented with insulin and IL-4. you end up with activated b cells expressing CD62L, CD83, CD86. they grow like gangbusters!

now to use this as antigen-presenting cells to detect antigen-specific T cells........

and it is spring here in Seattle, beautiful night last night. had a moonlight picnic down at The Cut.


Hmmm a biotech cell culture scientist living in Seattle ... I could make a pretty good guess where you work!

PM me sometime man!


Opening Day soon too
Rainiers.jpg
The following user(s) said Thank You: SunshineSue
iamthedoor
Platinum Boarder
Posts:1356

Re: Tell Me Something Good!!

#249181 1 year ago
Burt hooked me up with two tickets to DSO next Thursday in LA. A much needed date night for me and my lovely Brandi, not to mention our first DSO show! Now that's something good!
Warrior for Peace and The Positive Movement!
The following user(s) said Thank You: kevin
SunshineSue
Platinum Boarder
Posts:29212
Life is sweeter for this!

Re: Tell Me Something Good!!

#249189 1 year ago
iamthedoor wrote:
Burt hooked me up with two tickets to DSO next Thursday in LA. A much needed date night for me and my lovely Brandi, not to mention our first DSO show! Now that's something good!

He's going to ruin his reputation if he keeps being such a good guy all the time!!

Have fun at the show!
The following user(s) said Thank You: iamthedoor
Wundurland
Expert Boarder
Posts:620

Re: Tell Me Something Good!!

#249280 1 year ago
Game of Thrones is back on tomorrow night!
...was staring at the little blotch of sunshine with a special intensity, considering lying down in it.

-JD Salinger
The following user(s) said Thank You: SunshineSue
Ossumpei
Platinum Boarder
Posts:2602

Re: Tell Me Something Good!!

#249301 1 year ago
Once in a while my chili is pretty good.
Let us all look kindly on our neighbor.
The following user(s) said Thank You: SunshineSue
Ossumpei
Platinum Boarder
Posts:2602

Re: Tell Me Something Good!!

#249303 1 year ago
Reese's peanut butter cups are always good.
Let us all look kindly on our neighbor.
SunshineSue
Platinum Boarder
Posts:29212
Life is sweeter for this!

Re: Tell Me Something Good!!

#249315 1 year ago
What's really great is working outside in just a t-shirt!! For the first time in months it's sunny and 50's here, I'm loving it!!!
Ossumpei
Platinum Boarder
Posts:2602

Re: Tell Me Something Good!!

#249327 1 year ago
I just had some shrimp chow mein at my fab Chinese place, Wie Hong Bakery.

It was perfect, a 10 in all ways.

They've made this dish perfectly several times.

Now that was good.
Let us all look kindly on our neighbor.
The following user(s) said Thank You: SunshineSue, NobleGlassArt
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